Cas9 Proteins -Cas9 protein from Streptococcus pyogenes with NLS -Injection, transfection ready -Activity validated by in vitro cleavage assay, electroporation (as RNP complex), and mouse embryo -injection for each batch -Standard concentration (CP01, reconstitutes to 1 mg/ml): 20 ug, 50 ug, or 200 ug (50 ug x 4) -High concentration (CP02 & CP03, reconstitutes to 5 mg/ml): 250 ug (CP02) or 1 mg (CP03, 250 ug x 4) -Packaging: 20 ug or 50 ug for CP01 and 250 ug for CP02/CP03 -CP01 is recommended for microinjection and CP02/CP03 is recommended for electroporation -Also available: D10A Nickase (CN01) and D10A/H840A dCas9 (CD01) protein -Cas9 protein is usually shipped the same day if order is received by 5 pm EST
Liquid form of Cas9 protein -Cas9 protein variant with high fidelity without compromising on activity -Discovered by directed evolution method -Available as 1 mg/ml (CSP01), or 5 mg/ml (CSP02) -Read recent publication about SniperCas9
Cy3-Cas9 & Cy3-dCas9 protein -Cy3 labeled Cas9 protein with NLS (CP06) to enable sorting and visualization of transfected cells -Fluorescence detected by confocal microscopy -Activity confirmed by in vitro cleavage assay and cell transfection -Available as 50 ug (CP06-50) or 200 ug (CP06-200) at 5 mg/ml concentration -Also Cy3 labeled dCas9 protein (CD07) is available
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